IHepcomf
Plasma DNA Methylation Detection Kit for Liver Cancer
IHepcomf is a blood test for the qualitative detection of Hepatocellular carcinoma associated DNA methylated GNB4 and Riplet in plasma circulating cell-free DNA.
Studies have shown that cfDNA of cancer patients contains DNA fragments produced by tumor cell apoptosis, nerosis or secretion, and detecting cfDNA in plasma can distinguish cancer patients from healthy people.
The Progress of Liver Cancer
Why GNB4 and Riplet?
GNB4 and Riplet
The combined determination of GNB4 and Riplet showed excellent performance in the detection of clinical samples of liver cancer.
GNB4 is a key subunit of heterotrimeric G protein plays an important role in the occurrence and development of various malignant tumors. Studies have confirmed that the methylation of this gene is highly related to the occurrence of liver cancer
Riplet is a ubiquitin ligase that promotes retinoic acid-inducible gene I-mediated activation of the interferon beta promoter and inhibits the proliferation of negative-strand RNA viruses. Studies have shown that the methylation of this gene is a molecular marker for hepatocellular carcinoma.
Who's at risk?
With type diabetes
With liver cirrhosis
With chronic hepatitis
Chronic alcohol consumption
With a family history of liver cancer
With non-alcoholic fatty liver disease
The Advantage of IHepcomf
Non-invasive
Use blood as sample for detection.
Ultra sensitivity
Sensitivity: 93.54%
Specificity: 92.72%
Sensitivity is 30% better than ultrasound and AFP.
Fast result
Results available within 4.5h since sample processing
Simple testing procedure
Familiar Real-time PCR technology, compatible with mainstream brand instruments
Cost effective
The detection cost is lower than NGS
Detecting at early stage
Research have shown that changes in DNA methylation of tumor suppressor genes are a manifestation of tumors at an early stage
Kit Components of IHepcomf
Plasma cfDNA Extraction and Bisulfite Conversion Kit
Plasma DNA Methylation Detection Kit for Liver Cancer (Real-Time PCR)
Testing Procedures
Sample collection
Pretreatment and extraction
Bisulfite conversion and purification
qPCR procedure
Analysis and Report
Sample collection
Pretreatment and extraction
Bisulfite conversion and purification
qPCR procedure
Analysis and Report
Result Interpretation
It is recommended that patients with positive result should to go to the gastroenterology, hepatology or oncology hospital in time for gastrointestinal tumor-related examinations, such as imaging tests and other tests recommended by doctors.
Gene methylation |
| |
| GNB4 | Riplet | |
| Positive | Positive |
|
| Negative | Positive | |
| Positive | Negative | |
Negative | Negative |
|